Ce Ar細胞
運輸方式: 凍存運輸
數量: 大量
生長狀態: 貼壁生長
ATCC Number: CRL-1165?
相關**: 著色性干皮病
細胞形態: 成纖維樣
是否是腫瘤細胞: 0
物種來源: 人
年限: 54 years
器官來源: 皮膚
Designations: Ce Ar
Ce Ar細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: xeroderma pigmentosum, presumed heterozygote
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Ce Ar細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 54 years
Gender: female
Comments: The tissue donor was a presumed heterozygote for xeroderma pigmentosum, and was the mother of Ge Ar (ATCC CRL-1161) and Em Ar (ATCC CRL-1168).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Ce Ar細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Ce Ar細胞Subculture every 7 to 9 days.