Mar Nol細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 15 years
數(shù)量： 大量
器官來源： 皮膚
ATCC Number： CRL-1257?
相關(guān)**： 馬凡氏綜合征
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 成纖維樣
Mar Nol細(xì)胞是否是腫瘤細(xì)胞： 0
物種來源： 人
Designations: Mar Nol
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Mar Nol細(xì)胞Marfan syndrome
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 15 years
Gender: female
Comments: The tissue donor suffered from Marfan syndrome and floppy mitral valve syndrome.
There was no prior family history of Marfan syndrome.
Propagation: Mar Nol細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Mar Nol細(xì)胞Subculture every 8 to 10 days.
