CCD-1137Sk細胞
細胞類型： 成纖維細胞
是否是腫瘤細胞： 0
物種來源： 人
運輸方式： 凍存運輸
ATCC Number： CRL-2703?
器官來源： 皮膚
生長狀態： 貼壁生長
年限： newborn
細胞形態： 成纖維樣
數量： 大量
CCD-1137Sk細胞Designations: CCD-1137Sk
Depositors: F.Haq, ATCC
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: fibroblast
CCD-1137Sk細胞
Source: Organ: skin; foreskin
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: CCD-1137Sk細胞newborn
Gender: male
Comments: The line was established from skin taken from normal foreskin.
Cells senesce after approximately 60 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: CCD-1137Sk細胞Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
