4D12細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
運輸方式： 凍存運輸
數量： 大量
細胞形態： **樣
**類型： IgG1
ATCC Number： HB-178?
Designations: 4D12
Depositors: BF Haynes
Isotype: IgG1
4D12細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
4D12細胞B
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA B5
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: 4D12細胞Animals were immunized with the HUT 78 Sezary cutaneous T cell line.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with an HLA determinant that includes specificities within, but not identical to, the B5 cross-reactive group.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1193: Haynes BF, et al. Description of monoclonal antibody defining an HLA allotypic determinant that includes specificities within the B5 cross-reacting group. Hum. Immunol. 4: 273-285, 1982. 4D12細胞PubMed: 6181035
