Hs 895.Sk細(xì)胞
器官來源： 其他
是否是腫瘤細(xì)胞： 0
物種來源： 人
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-7636?
相關(guān)**： 正常
年限： 48 years
細(xì)胞形態(tài)： 成纖維樣
數(shù)量： 大量
運輸方式： 凍存運輸
Hs 895.Sk細(xì)胞Designations: Hs 895.Sk
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin (The line was established from apparently normal tissue from a patient who had metastatic melanoma.)
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis:Hs 895.Sk細(xì)胞 modal number = 46; range = 32 to 46
Age: 48 years
Gender: female
Ethnicity: Caucasian
Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
This normal skin fibroblast cell line was isolated from the same patient as Hs 895.T (ATCC CRL-7637), a melanoma. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Propagation: Hs 895.Sk細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to three days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: derived from same individual: Hs 895.Sk細(xì)胞ATCC CRL-7637, Hs 895
