LTPA細胞
品系： LT/Sv
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態： 上皮樣
ATCC Number： CRL-2389?
相關**： 腺癌
年限： 12 month old
運輸方式： 凍存運輸
器官來源： 胰腺
生長狀態： 貼壁生長
LTPA細胞數量： 大量
Designations: LTPA
Depositors: EH Leiter
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as Mus musculus (mouse)
Morphology: epithelial
Source: Organ: pancreas
Strain: LT/Sv
Disease: adenocarcinoma
LTPA細胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Age: 12 month old
Gender: female
Comments: LTPA is an epithelial cell line derived in 1975 by Edward H. Leiter at The Jackson Laboratory, Bar Harbor, Maine from a spontaneous pancreatic adenocarcinoma taken from a 12 month old female Lt/Sv mouse.
A defective Polyoma virus infection of a pancreatic duct cell or its precursor appears to represent the neoplastic transforming event. LTPA cells carry a persistent Polyoma infection.
When injected subcutaneously into Swiss nu/nu mice, LTPA cells formed ductular structures, which were destroyed by inflammatory reactions within 3 weeks.
LTPA細胞A culture submitted to the ATCC in May of 1998 was found to be contaminated with mycoplasma and progeny were cured by a 21-day treatment with BM Cycline.
The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. LTPA細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 38749: Leiter EH, et al. An epithelial cell line with chronic polyoma infection established from a spontaneous mouse pancreatic adenocarcinoma. Cancer Res. 38: 969-977, 1978. PubMed: 205354
