J.RT3-T3.5細胞
生長狀態： 懸浮生長
ATCC Number： TIB-153?
數量： 大量
相關**： 白血病
運輸方式： 凍存運輸
細胞形態： **樣
細胞類型： T**細胞
是否是腫瘤細胞： 0
物種來源： 人
Designations: J.RT3-T3.5
Depositors: A Weiss
J.RT3-T3.5細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: acute T cell leukemia
Cell Type: T lymphocyte;
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Applications: transfection host
Gender: male
Comments: J.RT3-T3.5細胞This is a mutant line derived from the E6-1 clone of Jurkat (ATCC TIB 152) that lacks the beta chain of the T cell antigen receptor. The cells do not express either CD3 or the T cell receptor alpha/beta heterodimer on the surface.
The line was produced by treatment with ethylmethanesulfonate and negative selection with OKT3 monoclonal antibody.
The cells may be useful for transfection studies involving the T cell receptor beta chain gene.
The J.RT3-T3.5 cell line is a derivative mutant of the Jurkat leukemia cell line.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. [50685] [112530]
J.RT3-T3.5 cells lack the beta chain of the T cell antigen receptor.
They do not express CD3 and and do not produce T cell receptor alpha/beta heterodimer.
The line may be useful in transfection studies of the T cell receptor alpha and beta chain genes.
Acute T cell leukemia.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: J.RT3-T3.5細胞Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow the cell concentration to exceed 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC TIB-152
References: 1819: Ohashi PS, et al. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature 316: 606-609, 1985. PubMed: 4033759
23436: Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306
50685: Schneider U, et al. J.RT3-T3.5細胞Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013
112530: Ronald Wange, personal communication
