NN-4細胞
ATCC Number： HB-8473?
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
細胞形態： **樣
**類型： IgG2a; kappa light chain
生長狀態： 懸浮生長
數量： 大量
NN-4細胞Designations: NN-4
Depositors: Lawrence Livermore National Lab
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: NN-4細胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human glycophorin A type N
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Comments: Animals were immunized with type O, Rh- human erythrocytes and purified human glycophorin A.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Propagation: NN-4細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: NN-4細胞1098: Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283
