LBRM-33-1A5細胞
品系： B10.BR
ATCC Number： CRL-8079?
數量： 大量
相關**： **瘤
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
生長狀態： 懸浮生長
器官來源： 脾
細胞形態： **樣
Designations: LBRM-33-1A5
LBRM-33-1A5細胞Depositors: S Gillis
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Strain: B10.BR
Organ: spleen
LBRM-33-1A5細胞Disease: lymphoma
Cellular Products: interleukin-2 (interleukin 2, IL-2)
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Receptors: interleukin 1 (IL-1)
Comments: The cells produce IL-2 in response to IL-1, and can be used to assay IL-1 activity.
Propagation: LBRM-33-1A5細胞ATCC complete growth medium: RPMI 1640 medium with 0.0025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Maintain at approximately 8 X 10 exp5 viable cells/ml.
References: 3528: Gillis S. Process for preparing murine interleukin-2 in the presence of interleukin-1 from an interleukin-2 nonproducer malignant cell line and cell line therefor. LBRM-33-1A5細胞US Patent 4,404,280 dated Sep 13 1983
