MAC 2-48細(xì)胞
細(xì)胞形態(tài): **樣
**類型: IgG1
運(yùn)輸方式: 凍存運(yùn)輸
生長(zhǎng)狀態(tài): 懸浮生長(zhǎng)
ATCC Number: HB-10714?
是否是腫瘤細(xì)胞: 0
物種來源: 小鼠
數(shù)量: 大量
Designations: MAC 2-48
MAC 2-48細(xì)胞Depositors: P Guyre, PM Morganelli
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: MAC 2-48細(xì)胞immunoglobulin; monoclonal antibody
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Comments: Animals were immunized with human monocytes.
MAC 2-48細(xì)胞Spleen cells were fused with NS-1 myeloma cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. MAC 2-48細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.