GH3細胞
年限： 7 months
細胞形態： 上皮樣
ATCC Number： CCL-82.1?
相關**： 腫瘤
數量： 大量
是否是腫瘤細胞： 0
物種來源： 大鼠
器官來源： 垂體
品系： Wistar-Furth
生長狀態： 漂浮生長
運輸方式： 凍存運輸
GH3細胞Designations: GH3
Depositors: AH Tashjian
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: loosely adherent with floating clusters
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: pituitary
Strain: Wistar-Furth
Disease: tumor
Cellular Products: prolactin; growth hormone (somatotrophin)
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Isolation: Isolation date: July, 1965
Applications: transfection host
Virus Susceptibility: Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1
Cytogenetic Analysis: modal number = 67; range = 47 to 71.
Stemline karyotype is stable with a few structural alterations. Two dicentric marker chromosomes were observed in 100% of the cells examined.
Age: 7 months
Gender: female
Comments: Clone GH3 was established in July, 1965 by A.H. Tashjian, Jr., et al. from a pituitary tumor carried in a 7-month-old female Wistar-Furth rat
Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established.
The epithelial-like GH3 clone generates growth hormone at a greater rate than the GH1 cells and also produces prolactin.
Studies on the control of the production of these protein hormones by the GH3 cells have shown that hydrocortisone stimulates the production of growth hormone and inhibits prolactin production.
The cells have been adapted to growth in suspension culture using Eagle's minimum essential medium (spinner) supplemented with 15% horse serum and 2.5% FBS.GH3細胞 Under these conditions the cells continue to produce both growth hormone and prolactin.
The cell line was submitted to the American Type Culture Collection in October, 1972 in the 10th subculture generation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it.
Add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin- 0.53 mM-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
derivative:GH3細胞ATCC CCL-82.2
References: 880: Bancroft FC, et al. Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone. J. Cell Biol. 43: 432-441, 1969. PubMed: 5389137
881: Tashjian AH Jr., et al. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281
883: Tashjian AH Jr., et al. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells. J. Cell Biol. 47: 61-70, 1970. PubMed: 5513559
884: Bancroft FC, Tashjian AH Jr.. Growth in suspension culture of rat pituitary cells which produce growth hormone and prolactin. Exp. Cell Res. 64: 125-128, 1971. PubMed: 5541958
2146: Bancroft FC, Tashjian AH Jr.. Control of the production of two protein hormones by rat pituitary cells in culture. In Vitro 6: 180-189, 1970. PubMed: 5535574
33124: Wood WM, et al. Thyroid hormone receptor beta2 promoter activity in pituitary cells is regulated by Pit-1. J. Biol. Chem. 271: 24213-24220, 1996. PubMed: 8798664
