BBM.1細胞
生長狀態： 懸浮生長
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-28?
細胞形態： **樣
**類型： IgG2b
Designations: BBM.1
Depositors: P Parham
Isotype: IgG2b
BBM.1細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human Beta-2 microglobulin; cytotoxic
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: BBM.1細胞Animals were immunized with MOLT-4 cells (ATCC CRL-1582, a human T cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 917: BBM.1細胞Brodsky FM, et al. Characterization of a monoclonal anti-B2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur. J. Immunol. 9: 536-545, 1979. PubMed: 91522
985: Parham P, et al. Arginine 45 is a major part of the antigenic determinant of human beta2-microglobulin recognized by mouse monoclonal antibody BBM.1. J. Biol. Chem. 258: 6179-6186, 1983. BBM.1細胞PubMed: 6189821
