B104-1-1細(xì)胞
年限： embryo
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 貼壁生長
品系： NIH/Swiss
ATCC Number： CRL-1887?
運(yùn)輸方式： 凍存運(yùn)輸
器官來源： 胚胎
數(shù)量： 大量
B104-1-1細(xì)胞細(xì)胞形態(tài)： 成纖維樣
Designations: B104-1-1
Depositors: RA Weinberg
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Organ: embryo
Strain: B104-1-1細(xì)胞NIH/Swiss
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments: This line was established by A.L. Schechter et al. in 1984 by transfecting NIH/3T3 cells with EcoR1 digested DNA from the rat neuroblastoma cell line B-104.
The cells contain the neu transforming gene which codes for a 185000 dalton antigen designated p185.
The p185 protein is strongly associated with the presence of glioblastoma and neuroblastoma oncogenes.
The neu oncogene is homologous to the erb-B oncogene, and p185 is serologically similar to the epidermal growth factor receptor.
Propagation: B104-1-1細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.
References: 22917: Schechter AL, et al. B104-1-1細(xì)胞The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109
