MC-SV-HUC T-2細胞
生長狀態： 貼壁生長
器官來源： 輸尿管
ATCC Number： CRL-9519?
運輸方式： 凍存運輸
年限： 11 years
細胞形態： 上皮樣
數量： 大量
組織來源： uroepithelium
是否是腫瘤細胞： 0
物種來源： 人
Designations: MC-SV-HUC T-2
MC-SV-HUC T-2細胞Depositors: Wisconsin Alumni Res. Fndn.
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: ureter
Tissue: uroepithelium
Cellular Products: uroepithelial keratins
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Tumorigenic: MC-SV-HUC T-2細胞Yes
Age: 11 years
Gender: male
Comments: The cells were initially transformed with SV40 virus, and later treated with 3-methylcholanthrene (MCA).
While the parental line (SV-HUC-1, see ATCC CRL-9520) was not tumorigenic and had a balanced chromosome composition, the MCA treated line became tumorigenic and aneuploid.
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.
Stress (such as chemical exposure) could possibly activate the virus.
Propagation:MC-SV-HUC T-2細胞 ATCC complete growth medium: Ham's F12 medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. MC-SV-HUC T-2細胞US Patent 4,980,290 dated Dec 25 1990
