Ep-16細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
ATCC Number： HB-155?
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgM; kappa light chain
Designations: Ep-16
Depositors: AW Hamburger
Isotype: IgM; kappa light chain
Ep-16細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human epithelium
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Comments: Ep-16細(xì)胞Animals were immunized with a membrane enriched extract of human foreskin keratinocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with an epithelial specific antigen on human foreskin keratinocytes but does not react with mesodermal cells or lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Ep-16細(xì)胞Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
References: 1003: Hamburger AW, et al. Isolation and characterization of a monoclonal antibody specific for epithelial cells. Cancer Res. 45: 783-790, 1985. Ep-16細(xì)胞PubMed: 2578308
