3197-3 (3T3-MSV)細胞
運輸方式： 凍存運輸
器官來源： 胚胎
生長狀態： 貼壁生長
細胞形態： 成纖維樣
細胞類型： 成纖維細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
數量： 大量
3197-3 (3T3-MSV)細胞年限： embryo
ATCC Number： CRL-1568?
Designations: 3197-3 (3T3-MSV)
Depositors: JE DeLarco
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Organ: embryo
Cell Type: fibroblast
Cellular Products: 3197-3 (3T3-MSV)細胞The cells release a growth factor in serum free medium.
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Age: embryo
Comments: The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
They do not produce virus particles.
The cells have a tendency to slough off the flask surface.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 3197-3 (3T3-MSV)細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 95%; bovine calf serum, 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin solution for 5 minutes, add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days.
References: 23261: de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512
26089: Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. 3197-3 (3T3-MSV)細胞PubMed: 4319233
