ESK-4細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 豬
年限： embryo
數(shù)量： 大量
ATCC Number： CL-184?
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
器官來(lái)源： 腎臟
Designations: ESK-4
Depositors: Syntex (USA), Inc.
ESK-4細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Sus scrofa
Morphology:
Source: Organ: kidney
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: Bovine viral diarrhea virus 1
Cytogenetic Analysis: normal female; diploid
Age: ESK-4細(xì)胞embryo
Gender: female
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA, remove trypsin and let the flask sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
ESK-4細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Storage temperature: liquid nitrogen vapor phase
References: 3523: Bordt DE, Thomas PC. Diploid porcine embryonic cell strains, cultures produced therefrom, and use of said cultures for production of vaccines. ESK-4細(xì)胞US Patent 4,070,453 dated Jan 24 1978
