RmcB細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
ATCC Number： CRL-2379?
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgG1
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
Designations: RmcB
RmcB細(xì)胞Depositors: RW Finberg
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human CAR (Coxsackie - Adenovirus Receptor)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: RmcB細(xì)胞A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells. [38635]
Spleen cells were fused with Sp2/0 myeloma cells.
The RmcB cell line was established in 1985 by R. L. Crowell. [38636]
The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3). [29621]
The antibody can block binding of adenovirus and block binding and infection by coxsackie B Virus.
Mouse monoclonal antibody (RmcB) protects HeLa cells and Buffalo green monkey kidney cells from infection by all six serotypes of CB. RmcB did not protect HeLa cells from infection by poliovirus, echovirus 6, or coxsackievirus A18. [38636]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cultures between 1 X 10 exp5 and 9 X 10 exp5 cells/ml.
RmcB細(xì)胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 29621: Bergelson JM, et al. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275: 1320-1323, 1997. PubMed: 9036860
38635: Mapoles JE, et al. Purification of a HeLa cell receptor protein for group B coxsackieviruses. J. Virol. 55: 560-566, 1985. PubMed: 2991580
38636: Hsu KH, et al. RmcB細(xì)胞A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses. J. Virol. 62: 1647-1652, 1988. PubMed: 2451756
