RK3E細胞
數量： 大量
器官來源： 腎臟
ATCC Number： CRL-1895?
相關**： 正常
年限： 7 days
運輸方式： 凍存運輸
細胞類型： 其他細胞類型
生長狀態： 貼壁生長
品系： Fischer
是否是腫瘤細胞： 0
RK3E細胞物種來源： 大鼠
細胞形態： 上皮樣
Designations: RK3E
Depositors: K Kinzler, B Vogelstein, J Ruppert
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Strain: Fischer
Organ: kidney
RK3E細胞Disease: normal
Cell Type: E1A immortalized
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Applications: transfection host
Tumorigenic: No
Age: 7 days
Comments: The RK3E cell line was derived by immortalizing rat kidney cells with the E1A oncogene.
The cells can be transformed with the RAS and GLI oncogenes.
The cells have an essentially normal karyotype with the exception of one 5q.
Transformation with RAS or GLI renders the cells tumorigenic.
Propagation: RK3E細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove old medium, and add fresh 0.25% trypsin, 0.03% EDTA solution. Let sit 2 minutes, remove trypsin and allow the flask to incubate at 37C for 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22382: Kinzler KW, et al. Identification of an amplified, highly expressed gene in a human glioma. Science 236: 70-73, 1987. PubMed: 3563490
22576: Kinzler KW, Vogelstein B. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol. Cell. Biol. 10: 634-642, 1990. PubMed: 2105456
24394: Kinzler KW, et al. RK3E細胞The GLI gene is a member of the Kruppel family of zinc finger proteins. Nature 332: 371-374, 1988. PubMed: 2832761
