39-S細胞
ATCC Number: HB-8180?
數量: 大量
細胞形態: **樣
**類型: IgG2a
生長狀態: 懸浮生長
運輸方式: 凍存運輸
是否是腫瘤細胞: 0
物種來源: 小鼠
Designations: 39-S
39-S細胞Depositors: The United States of America
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) internal capsid protein 8 (ICP 8)
Permits/Forms: 39-S細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with HSV-1.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody is type specific for HSV-1 but does not neutralize the virus.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15% -OR- RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing: 39-S細胞Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1178: Showalter SD, et al. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infect. Immun. 34: 684-692, 1981. PubMed: 6277788
3544: Zweig M, et al. Monoclonal antibodies to herpes simplex virus type 1 polypeptides. 39-S細胞US Patent 4,572,896 dated Feb 25 1986