SJD.1細胞

ATCC Number： CRL-2296?

細胞形態： 成纖維樣

數量： 大量

生長狀態： 貼壁生長

是否是腫瘤細胞： 0

物種來源： 斑馬魚

器官來源： 其他

品系： SJD

年限： *****

運輸方式： 凍存運輸

Designations: SJD.1

SJD.1細胞Depositors: BH Paw, LI Zon

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Danio rerio

Morphology: fibroblast

Source: Organ: caudal fin

Strain: SJD

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Age: *****

Comments: SJD.1細胞SJD.1 is a fibroblast cell line derived from amputated caudal fins of an ***** zebrafish, strain SJD.

This strain of zebrafish is used for genetic mapping.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.

Temperature: 28.0°C

SJD.1細胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, rinse with trypsin (0.25%)-EDTA (0.03%)-polyvinylpyrrolidone (0.5%) solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature until cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: SJD.1細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 43315: Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354