SH3細(xì)胞
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： HB-10744?
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
**類型： IgG2b
Designations: SH3
SH3細(xì)胞Depositors: Osiris Therapeutics, Inc.
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human mesenchymal stem cells
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SH3細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with human mesenchymal stem cells.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody reacts with mesenchymal stem cells from bone marrow and some tissues of mesenchymal origin.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
SH3細(xì)胞Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996
49752: Haynesworth SE, et al. SH3細(xì)胞Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137
