El Don細胞
ATCC Number： CRL-1149?
相關**： 其他**
年限： 31 years
運輸方式： 凍存運輸
生長狀態(tài)： 貼壁生長
器官來源： 皮膚
是否是腫瘤細胞： 0
物種來源： 人
El Don細胞數(shù)量： 大量
細胞形態(tài)： 成纖維樣
Designations: El Don
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: El Don細胞Ehlers-Danlos syndrome, type VII (arthrocalasis type)
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Age: 31 years
Gender: female
Comments: The tissue donor suffered from type VII Ehlers-Danlos syndrome (arthrocalasis type), and was patient No. 2 in Lichtenstein, JR, et al. Science 182:298, 1973.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. El Don細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.
References: 22242: Lichtenstein JR, et al. El Don細胞Defect in conversion of procollagen to collagen in a form of Ehlers-Danlos syndrome. Science 182: 298-300, 1973. PubMed: 4742738
