EH7a細胞
細胞形態： **樣
**類型： IgG1
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
生長狀態： 懸浮生長
數量： 大量
ATCC Number： CRL-2194?
EH7a細胞Designations: EH7a
Depositors: JD Ernst
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:EH7a細胞 immunoglobulin; monoclonal antibody; against human annexin I and human annexin II
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Tumorigenic: Yes
Comments: Animals were immunized with a mixture of Ca++ dependent phospholipid binding proteins isolated from human polymorphonuclear leukocytes.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody recognizes human annexin I amino acids 41 - 118, and inhibits annexin I mediated phospholipid vesicle aggregation.
Annexin II mediated vesicle aggregation is not affected by EH7a antibody.
Propagation: EH7a細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23407: Ernst JD, et al. Identification of a domain that mediates vesicle aggregation reveals functional diversity of annexin repeats. J. Biol. Chem. 266: 6670-6673, 1991.EH7a細胞PubMed: 1707872
