SE-1.3細胞
ATCC Number： HB-137?
生長狀態： 懸浮生長
數量： 大量
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態： **樣
**類型： IgE
SE-1.3細胞Designations: SE-1.3
Depositors: A Nisonoff, S Haba
Isotype: IgE
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: SE-1.3細胞immunoglobulin; monoclonal antibody; against phenylarsonate
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Comments: Spleen cells from normal A/J were fused with Sp2/0-Ag14 myeloma cells.
Periodic recloning is recommended if the cells are to be maintained in continuous culture.
Tested and found negative for ectromelia virus (mousepox).
Propagation: SE-1.3細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium SE-1.3細胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X
