SF1-1.1.10細胞
運輸方式： 凍存運輸
數(shù)量： 大量
ATCC Number： HB-159?
生長狀態(tài)： 懸浮生長
細胞形態(tài)： **樣
**類型： IgG2a
是否是腫瘤細胞： 0
物種來源： 小鼠
Designations: SF1-1.1.10
SF1-1.1.10細胞Depositors: AM Stall
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: SF1-1.1.10細胞immunoglobulin; monoclonal antibody; against H-2 K d
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with spleen cells from BALB/c mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody does not react with the b, f, k, p, q, s or v H-2 haplotypes.
SF1-1.1.10細胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. SF1-1.1.10細胞Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
