Schneider's Drosophila Line 2細(xì)胞
器官來(lái)源： 胚胎
年限： embryo; 20 to 24 hours
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-1963?
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 果蠅
Schneider's Drosophila Line 2細(xì)胞細(xì)胞形態(tài)： 上皮樣
Designations: Schneider's Drosophila Line 2 [D. Mel. (2), SL2]
Depositors: IR Schneider
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension or loosely adherent
Organism: Drosophila melanogaster
Morphology: epithelial
Source: Organ: embryo
Permits/Forms: Schneider's Drosophila Line 2細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1969
Applications: transfection host (Roche Transfection Reagents)
Age: embryo; 20 to 24 hours
Comments: The cells have been shown to support the growth of the insect stages of malaria parasites [Pubmed ID: 1734521].
Propagation: ATCC complete growth medium: Schneider's Drosophila Medium, 90%; heat-inactivated fetal bovine serum, 10%. NOTE: the fetal bovine serum should be selected for ability to support the growth of insect cell lines.
This medium is formulated for use without CO2.
Temperature: Schneider's Drosophila Line 2細(xì)胞23.0°C; (Max. 24.0 °C, Min. 22.0°C)
Note: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16.0°C and 28.0°C.
Subculturing: Protocol: Gently resuspend the cells by pipetting medium across the monolayer or (for larger flasks) by slapping the flask against the base of the palm. Dilute the cell suspension to the desired concentration with fresh medium.
Maintain cultures at a cell concentration between 5 X 10(4) and 4 X 10(5) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:10 or greater is recommended
Medium Renewal: At the time of subcultivation
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: approximately 67 hours
References: 22960: Warburg A, Miller LH. Schneider's Drosophila Line 2細(xì)胞Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521
22983: Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27: 353-365, 1972. PubMed: 4625067
