SCP細胞
ATCC Number： CRL-1700?
運輸方式： 凍存運輸
組織來源： choroid plexus
器官來源： 大腦
數量： 大量
是否是腫瘤細胞： 0
物種來源： 綿羊
生長狀態： 貼壁生長
Designations: SCP
Depositors: W Siegel, T Booker
SCP細胞Biosafety Level: 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Ovis aries
Morphology:
Source: Organ: brain
Tissue: choroid plexus
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Virus Susceptibility: Visna virus (strain 1514)
Comments: SCP細胞The line is positive for bovine viral diarrhea virus (BVD).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: SCP細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
32728: Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. SCP細胞PubMed: 8523528
