RDP 45/20細胞
ATCC Number： TIB-98?
細胞形態： **樣
**類型： IgM
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
生長狀態： 懸浮生長
數量： 大量
Designations: RDP 45/20
RDP 45/20細胞Depositors: WC Raschke
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; RDP 45/20細胞monoclonal antibody; against the Igh-1b allotypic determinant on mouse IgG2a
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with CBPC-101 myeloma protein [IgG2a (Ig-1b)].
Spleen cells were fused with the MPC-11 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: RDP 45/20細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 2127: DiPauli R, Raschke WC. RDP 45/20細胞A hybridoma secreting IgM anti-IgIb allotype. Curr. Top. Microbiol. Immunol. 81: 37-39, 1978. PubMed: 567562
