SC細胞
器官來源： 外周血
數量： 大量
細胞形態： 單核細胞/巨噬細胞
生長狀態： 懸浮生長
ATCC Number： CRL-9855?
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 人
SC細胞運輸方式： 凍存運輸
Designations: SC
Depositors: E.I. du Pont de Nemours & Co.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: monocyte/macrophage
Source: Organ: peripheral blood
Cell Type: SC細胞monocyte/macrophage
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Comments: The SC cell line was established from human peripheral blood mononuclear cells.
The cells grow as single cells in suspension, and have a granular appearance.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium.
Interval: SC細胞Maintain the cell concentration between 2 X 10(5) and 1 X 10 (6) cells/ml)
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 26300: Collins GW, Largen MT. SC細胞Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995
