E (Ehrlich-Lettre ascites)細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 貼壁生長

ATCC Number： CCL-77?

數(shù)量： 大量

E (Ehrlich-Lettre ascites)細(xì)胞細(xì)胞形態(tài)： 上皮樣

Designations: E (Ehrlich-Lettre ascites)

Depositors: CW Boone

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Permits/Forms: E (Ehrlich-Lettre ascites)細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 3

Comments: Tested and found negative for ectromelia virus (mousepox).

E (Ehrlich-Lettre ascites)細(xì)胞Propagation: ATCC complete growth medium: NCTC 135 medium, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Subcultures can be prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Alternatively, a standard trypsinization procedure can be used.

References: 22170: Boone C, et al. Characterization of an in vitro strain of Ehrlich-Lettre ascites carcinoma subjected to many periodic mouse passages. J. Natl. Cancer Inst. 34: 725-740, 1965. E (Ehrlich-Lettre ascites)細(xì)胞PubMed: 5829538