UV135細胞
是否是腫瘤細胞： 0
物種來源： 倉鼠
器官來源： 卵巢
運輸方式： 凍存運輸
ATCC Number： CRL-1867?
細胞形態： 上皮樣
生長狀態： 單層、懸浮
數量： 大量
Designations: UV135 (UV sensitive mutant of CHO)
Depositors: LH Thompson
Biosafety Level: 1
UV135細胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: monolayer and suspension
Organism: Cricetulus griseus
Morphology: epithelial-like
Source: Organ: ovary
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: female
Comments: This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).
UV135 is a UV sensitive line derived from AA8 (see ATCC CRL-1859).
The line is defective in nucleotide excision repair, is sensitive to bulky adduct mutagens and belongs to excision repair complementation group 5.
Propagation: UV135細胞ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
Medium Renewal: 2 to 3 times per week
To subculture attached cells, remove the medium and rinse the cells with fresh 0.25% trypsin, 0.03% EDTA.
Let the culture sit at 37C until the cells detach (about 5 minutes).
Add fresh medium, aspirate and transfer to new flasks.
The suspended cells are viable and can be used to start new cultures.
Doubling Time: 13 hrs
References: 1767: Thompson LH, et al. Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene. Somatic Cell Mol. Genet. 10: 183-194, 1984. PubMed: 6584989
1768: Thompson LH, et al. Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78: 3734-3737, 1981. PubMed: 6943579
1769: Hoy CA, et al. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents. Cancer Res. 45: 1737-1743, 1985. PubMed: 3919945
1770: Busch D, et al.UV135細胞 Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628
21855: . Cellular responses to DNA damage. New York: Liss; 1983.
32479: Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913
32924: Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841
58395: Thompson LH, et al. UV135細胞Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. Somatic Cell Genet. 8: 759-773, 1982. PubMed: 7163954
