S6F1細胞 
	
		生長狀態:	懸浮生長
	
	
		細胞形態:	**樣
	
	
		**類型:	IgG1
	
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	小鼠
	
	
		ATCC Number:	HB-9579?
	
	
		運輸方式:	凍存運輸
	
	
		數量:	大量
	
	
		S6F1細胞Designations:	S6F1
	
	
		Depositors:	 Dana Farber Cancer Inst.
	
	
		Isotype:	IgG1
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	suspension
	
	
		Organism:	Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
	
	
		Morphology:S6F1細胞 lymphoblast
	
	
		
	
	
		Source:	Cell Type: hybridoma: B lymphocyte;
	
	
		Cellular Products:	immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Comments:	Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
	
	
		Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
	
	
		S6F1細胞The antibody recognizes a subset of CD8 positive T cells that have cytotoxic effector activity.
	
	
		The S6F1 epitope is absent on CD8 positive suppressor cells.
	
	
		Propagation:	ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.1 mM hypoxanthine, and 0.032 mM thymidine, 80%; heat-inactivated fetal bovine serum, 10%; heat-inactivated horse serum, 10%
	
	
		Subculturing:	Medium Renewal: Every 2 to 3 days
	
	
		Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
	
	
		References:	21964: S6F1細胞Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991