RAG細胞 
	
		品系:	BALB/c
	
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	小鼠
	
	
		器官來源:	腎臟
	
	
		ATCC Number:	CCL-142?
	
	
		數量:	大量
	
	
		相關**:	其他**
	
	
		細胞形態:	其他
	
	
		運輸方式:	凍存運輸
	
	
		生長狀態:	貼壁生長
	
	
		Designations:	RAG
	
	
		RAG細胞Depositors:	 RJ Klebe, FH Ruddle
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Mus musculus
	
	
		Morphology:	amoeboid
	
	
		
	
	
		Source:	Organ: kidney
	
	
		Strain: BALB/c
	
	
		Disease: renal adenocarcinoma
	
	
		Cellular Products:	kidney specific esterase-2 (ES-2)
	
	
		Permits/Forms: RAG細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Virus Resistance:	poliovirus 1
	
	
		Comments:	This is a non-reverting 8-azaguanine resistant (HPRT -, HGPRT -) clone of the Renal-2a cell line.
	
	
		Tested and found negative for ectromelia virus (mousepox).
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
	
	
		Medium Renewal: 3 times per week
	
	
		RAG細胞Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
	
	
		Add fresh culture medium, aspirate and dispense into new culture flasks.
	
	
		Preservation:	culture medium 95%; DMSO, 5%
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
	
	
		recommended serum:ATCC 30-2020
	
	
		References:	1025: Klebe RJ, et al. Mapping of a human genetic regulator element by somatic cell genetic analysis. Proc. Natl. Acad. Sci. USA 66: 1220-1227, 1970. PubMed: 4920091
	
	
		22239: . . Science 145: 709, 1964.
	
	
		22275: Klebe RJ, et al. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74-82, 1970. PubMed: 4318843
	
	
		22519: Felluga B, et al. Electron microscope observations on virus particles associated with a transplantable renal adenocarcinoma in BALB-cf-Cd mice. J. Natl. Cancer Inst. 43: 319-333, 1969. RAG細胞PubMed: 5797837