S49.1H.1AG.6/2細(xì)胞
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
數(shù)量： 大量
ATCC Number： TIB-29?
相關(guān)**： **瘤
生長狀態(tài)： 懸浮生長
品系： BALB/c
細(xì)胞形態(tài)： **樣
S49.1H.1AG.6/2細(xì)胞Designations: S49.1H.1AG.6/2
Depositors: M Cohn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. S49.1H.1AG.6/2細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2d; Thy-1.2; TL
Comments: The cells are resistant to 0.01 mM cortisol and 1 mM 8-azaguanine.
The line was derived from S49.1 (ATCC TIB-28) by selection for resistance to cortisol and 8-azaguanine.
Tested and found negative for ectromelia virus (mousepox).
S49.1H.1AG.6/2細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and maintain between 3 X 10 exp5 and 7 X 10 exp5 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22241: Schiller JG, et al. S49.1H.1AG.6/2細(xì)胞Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230
