R6.5.D6.E9.B2 [R6-5-D6]細(xì)胞
器官來源： 脾
生長狀態(tài)： 懸浮生長
ATCC Number： HB-9580?
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： mouse IgG2a kappa
年限： *****
運輸方式： 凍存運輸
R6.5.D6.E9.B2 [R6-5-D6]細(xì)胞品系： BALB/c
數(shù)量： 大量
Designations: R6.5.D6.E9.B2 [R6-5-D6]
Depositors: Dana Farber Cancer Inst.
Isotype: mouse IgG2a kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Strain: R6.5.D6.E9.B2 [R6-5-D6]細(xì)胞BALB/c
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: *****
Comments: Animals were immunized with the EBV transformed lymphoblast cell line, JY.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
R6.5.D6.E9.B2 [R6-5-D6]細(xì)胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 6 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with extra 10% FBS and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 12 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22051: Springer TA, et al. R6.5.D6.E9.B2 [R6-5-D6]細(xì)胞Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994
