UC7-13D5細胞
細胞形態(tài)： **樣
**類型： IgG
ATCC Number： CRL-1989?
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 倉鼠
Designations: UC7-13D5
UC7-13D5細胞Depositors: JA Bluestone
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. UC7-13D5細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for all murine TCR gamma/delta heterodimers.
UC7-13D5細胞Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: 90% complete growth medium, 10% DMSO.
References: 22362: Bluestone JA, et al. UC7-13D5細胞Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol. Rev. 120: 5-33, 1991. PubMed: 1650760
