R4-6A2細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 大鼠
生長狀態： 懸浮生長
數量： 大量
細胞形態： **樣
**類型： IgG1
ATCC Number： HB-170?
Designations: R4-6A2
R4-6A2細胞Depositors: GL Spitalny
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse gamma interferon
Permits/Forms:R4-6A2細胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with partially purified murine gamma interferon.
Spleen cells were fused with P3U-1 myeloma cells.
The antibody neutralizes the antiviral effect of gamma interferon but does not affect the activities of murine alpha and beta interferon or rat or human gamma interferon.
The antibody also neutralizes the ability of lymphokine preparations to induce tumoricidal activity in murine macrophages.
30 ng of R4-6A2 antibody neutralizes the antiviral activity of 10 units of murine gamma interferon.
Tested and found negative for ectromelia virus (mousepox).
Propagation: R4-6A2細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Do not allow the cell concentration to reach 1 x 10(6) cells/ml.
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10(4) cells/ml and maintain between 6 X 10(4) and 6 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Related Products:R4-6A2細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1174: Spitalny GL, Havell EA. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J. Exp. Med. 159: 1560-1565, 1984. PubMed: 6425450
27329: Tay CH, Welsh RM. Distinct organ-dependent mechanisms for the control of murine cytomegalovirus infection by natural killer cells. J. Virol. 71: 267-275, 1997. PubMed: 8985346
32947: Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913
32949: Skeen MJ, et al. Regulation of murine macrophage IL-12 production. J. Immunol. 156: 1196-1206, 1996. PubMed: 8557998
33098: Sayles PC, Johnson LL. R4-6A2細胞Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838
