219細胞
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
ATCC Number： HB-9371?
細胞形態： **樣
**類型： IgG2b
Designations: 219 [Anti PDGF Hybridoma #219]
219細胞Depositors: Amgen, Inc.
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; 219細胞monoclonal antibody; against platelet derived growth factor B chain (PDGF B, v-sis form)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with affinity purified PDGF B v-sis.
Spleen cells were fused with Sp2/0-Ag14 myeloma cell.
The antibody reacts with both the A and B chains of PDGF v-sis.
It cross-reacts with the platelet form of PDGF.
Propagation: 219細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: fetal bovine serum, 90%; DMSO, 10%
References: 41495: Thomason AR, Nicolson MA. 219細胞Methods for purification of platelet-derived growth factor. US Patent 5,175,255 dated Dec 29 1992
