225細胞

細胞形態： **樣

**類型： IgG1 , IgG1

生長狀態： 懸浮生長

數量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-8508?

運輸方式： 凍存運輸

Designations: 225

Depositors: Regents of the University of California

Isotype: IgG1 , IgG1

225細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor

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Tumorigenic: Yes

Comments: Animals were immunized with partially purified EGF receptors from A-431 cells.

Spleen cells were fused with NS-1-503 myeloma cells.

225細胞The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor and inhibits the growth of A-431 and T222 tumors in nude mice (although not as strongly as 528 (see ATCC HB-8509).

The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts, and it blocks EGF stimulated phosphorylation of membrane proteins.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; horse serum, 15%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. 225細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2040

References: 21948: Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990

92782: Sato JD, et al. 225細胞Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961