S1A(Thy-1-b)細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： TIB-231?
相關(guān)**： **瘤
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
S1A(Thy-1-b)細(xì)胞品系： BALB/c
Designations: S1A(Thy-1-b)
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: S1A(Thy-1-b)細(xì)胞T lymphocyte;
Cellular Products: The cells secrete a mutant Thy-1 glycoprotein.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
S1A(Thy-1-b)細(xì)胞The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1807: Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. S1A(Thy-1-b)細(xì)胞PubMed: 2895421
