2D4細胞
生長狀態： 懸浮生長
ATCC Number： TIB-185?
細胞形態： **樣
**類型： IgM
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
Designations: 2D4
2D4細胞Depositors: WW Young
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against asialo GM2
Permits/Forms: 2D4細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with asialo GM2 non-covalently adsorbed to naked Salmonella minnesota.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 2D4細胞ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 971: Young WW Jr., et al. Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (Asialo GM2). J. Exp. Med. 150: 1008-1019, 1979. PubMed: 512581
22944: Young WW J, Hakomori SI. Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo. Science 211: 487-489, 1981. 2D4細胞PubMed: 7455688
