R1.1細胞

細胞類型： T**細胞

生長狀態(tài)： 懸浮生長

是否是腫瘤細胞： 0

物種來源： 小鼠

數(shù)量： 大量

ATCC Number： TIB-42?

相關(guān)**： **瘤

運輸方式： 凍存運輸

品系： C58/J

細胞形態(tài)： **樣

R1.1細胞器官來源： 胸腺

Designations: R1.1

Depositors: P Ralph

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: thymus

Strain: C58/J

Disease: lymphoma

Cell Type: R1.1細胞T lymphocyte;

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Antigen Expression: H-2k; Thy-1.2; TL

Comments: Established from a spontaneous thymoma.

Sensitive to 0.001 mM cortisol and 10 mcg/ml PHA.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%

Subculturing: R1.1細胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

22247: Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. R1.1細胞PubMed: 4542714