2E.6細胞
是否是腫瘤細胞： 0
物種來源： 大鼠
數量： 大量
細胞形態： **樣
**類型： IgG1
運輸方式： 凍存運輸
ATCC Number： HB-128?
生長狀態： 懸浮生長
Designations: 2E.6
Depositors: JM Davie
2E.6細胞Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse 2E.6細胞(myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse IgG3
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: 2E.6細胞Animals were immunized with mouse myeloma proteins of the IgG1 (MOPC-21), IgG2a (HOPC-1), IgG2b (MPC-11), IgG3 (J606 and GAC-39) and IgM (M104E) subclasses.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with non-essential amino acids, 85%; horse serum, 10%; fetal bovine serum, 5%. If desired, the fetal bovine serum may be omitted
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 2E.6細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
