YAC-1細(xì)胞
數(shù)量： 大量
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
品系： A/Sn
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
ATCC Number： TIB-160?
相關(guān)**： **瘤
Designations: YAC-1
YAC-1細(xì)胞Depositors: JY Djeu
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: A/Sn
Cell Type: Moloney murine leukemia virus (Mo-MuLV) induced
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. YAC-1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: assay of NK cell activity ( [22345] [49299] The cells are sensitive to the action of natural killer (NK) cells and are useful in assays of NK cell activity.)
Comments: Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 2 X 10(5) and 2 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: YAC-1細(xì)胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 22345: Kiessling R, et al. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur. J. Immunol. 5: 112-117, 1975. PubMed: 1234049
26348: Cikes M, et al. Progressive loss of H-2 antigens with concomitant increase of cell- surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J. Natl. Cancer Inst. 50: 347-362, 1973. PubMed: 4573851
32552: Okazaki IJ, et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed: 8703012
49299: Kiessling R, et al. "Natural" killer cells in the mouse. II. YAC-1細(xì)胞Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur. J. Immunol. 5: 117-121, 1975. PubMed: 1086218
