2F.11.15細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： TIB-194?
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgA
數量： 大量
運輸方式： 凍存運輸
2F.11.15細胞Designations: 2F.11.15
Depositors: H Nariuchi
Isotype: IgA
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 2F.11.15細胞immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
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Comments: Animals were immunized with TNP-KLH.
2F.11.15細胞Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 2F.11.15細胞Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
