GeLu細(xì)胞

器官來源： 肺

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 貼壁生長

年限： 403 days

是否是腫瘤細(xì)胞： 0

物種來源： 其他

GeLu細(xì)胞ATCC Number： CCL-100?

Designations: GeLu

Depositors: ML Macy

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Meriones unguiculatus

Morphology: fibroblast

Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GeLu細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 1, 3

Age: 403 days

Gender: female

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin - 0.53 mM EDTA solution and remove. Place culture at room temperature until cells detach (approximately 10 to 20 minutes). Add fresh medium, aspirate and dispense into new flasks.

GeLu細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 26276: . . TCA GeLu細(xì)胞(Tissue Cult. Assoc.) Man. 3: 617-621, 1977.