2AB1-IA10細胞
生長狀態： 懸浮生長
數量： 大量
細胞形態： **樣
**類型： IgG2b
是否是腫瘤細胞： 0
物種來源： 大鼠
ATCC Number： HB-8210?
運輸方式： 凍存運輸
Designations: 2AB1-IA10
2AB1-IA10細胞Depositors: University of Pittsburgh
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: 2AB1-IA10細胞B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against laminin (basement membrane glycoprotein)
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Comments: Animals were immunized with extracellular matrix proteins from M1536-B3 cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
2AB1-IA10細胞The antibody reacts with laminin and can be used to detect laminin in tissues, and for isolation and quantitation of laminin.
Propagation: ATCC complete growth medium: Alpha minimum essential medium, 90%; heat-inactivated horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3880: Chung AE. 2AB1-IA10細胞Novel hybridoma cell line producing monoclonal antibodies against laminin and a process for the production thereof. US Patent 4,642,293 dated Feb 10 1987
