LS-136細胞
細胞形態： **樣
**類型： IgG1; kappa light chain
ATCC Number： TIB-157?
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
數量： 大量
生長狀態： 懸浮生長
LS-136細胞Designations: LS-136
Depositors: K Rajewsky
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type:LS-136細胞 hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain
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Comments: Animals were immunized with purified B1-8 monoclonal antibody.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein.
The antibody is specific for the C1 domain.
LS-136細胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 961: Reth M, et al. LS-136細胞Analysis of the repertoire of anti-(4-hydroxy-3-nitro-phenyl) acetyl (NP) antibodies in C57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9: 1004-1013, 1979. PubMed: 398773
