tk- ts13細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 其他
ATCC Number： CRL-1632?
生長狀態： 貼壁生長
器官來源： 腎臟
數量： 大量
Designations: tk- ts13
tk- ts13細胞Depositors: R Baserga
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mesocricetus auratus
Morphology:
Source: Organ: kidney
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. tk- ts13細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Can be transfected at a high rate of efficiency.
Comments: Thymidine kinase-deficient derivative of ts13, originally isolated as a temperature-sensitive mutant of the baby hamster kidney cell line BHK-21.
The tk-ts13 cell line is a derivative of BHK-21.
The cells are temperature sensitive for growth, and are thymidine kinase deficient (TK-).
The line is an efficient host for transfection studies.
The cells should be grown at 34C.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
tk- ts13細胞Temperature: 34.0°C
Subculturing: Remove medium, rinse with fresh 0.25% trypsin, 0.02% versene solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks Inoculate new cultures at 2 X 10 exp4 cells/sq cm.
References: 26118: Talavera A, Basilico C. Temperature sensitive mutants of BHK cells affected in cell cycle progression. J. Cell. Physiol. 92: 425-436, 1977. PubMed: 903382
32471: Black ME, et al. Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proc. Natl. Acad. Sci. USA 93: 3525-3529, 1996. PubMed: 8622970
41386: Shen YM, et al. tk- ts13細胞Superimposition of temperature regulation on yeast promoters. Mol. Cell. Biol. 2: 1145-1154, 1982. PubMed: 6294505
